cd69  (Bioss)

Journal: Frontiers in Immunology

Article Title: Single-cell and bulk transcriptomics reveal a CD8 + T-cell gene signature predicting prognosis in diffuse large B-cell lymphoma

doi: 10.3389/fimmu.2025.1685541

Figure Lengend Snippet: Validation cohort for predicting the risk signature of DLBCL survival based on the discovery cohort. (A) Expression of CD69 on infiltrating CD8 + T cells in DLBCL (400X). CD69 (red), CD8 (green), DAPI (blue). (B) Kaplan–Meier curves of OS in DLBCL patients with CD69 + /CD8 + and CD69 + /CD8 + . Cases were classified as CD69 + /CD8 + when ≥10% of infiltrating CD8 + T cells expressed CD69. (C) Expression of CD70 on infiltrating CD8 + T cells in DLBCL (400X). CD70 (green), CD8 (red), DAPI (blue). (D) Kaplan–Meier curves of OS in DLBCL patients with CD70 + /CD8 + and CD70 + /CD8 + . Cases were classified as CD70 + /CD8 + when ≥10% of infiltrating CD8 + T cells expressed CD70. (E) The Kaplan-Meier OS curve of the validation cohort (this work. (n=66)) patients between low risk group (n=34) and high risk group (n=32). This work samples were stratified by risk score. (F) Univariate Cox regression analysis of Risk Score: high risk, IPI: low-mid, IPI: mid-high and IPI: high in this work. (G) The Kaplan-Meier OS curve of the validation cohort ( GSE181063 (n=773)) patients between low risk group (n=387) and high risk group (n=386). GSE181063 samples were stratified by risk score. (H) Univariate Cox regression analysis of Risk Score: high risk, IPI: low-mid, IPI: mid-high and IPI: high in GSE181063 . (I) The Kaplan-Meier OS curve of the validation cohort ( GSE117556 (n=469)) patients between low risk group (n=235) and high risk group (n=234). GSE117556 samples were stratified by risk score. (J) Univariate Cox regression analysis of Risk Score: high risk, IPI: low-mid, IPI: mid-high and IPI: high in GSE117556 . Log-rank tests were used to derive p-values for comparisons between two groups.

Article Snippet: The primary antibodies are as follows: CD69 Polyclonal antibody (Proteintech, 10803-1-AP), CD70 Monoclonal antibody (Proteintech, 67749-1-Ig), CD8a Monoclonal antibody (Proteintech, 66868-1-Ig), Anti-CD8 alpha antibody (Abcam, ab93278).

Techniques: Biomarker Discovery, Expressing

Journal: Environment & Health

Article Title: Environmental Enrichment Exposure Alleviates Geriatric Depressive-Like Symptoms through Regulating Neurogenesis and Neuroinflammation

doi: 10.1021/envhealth.4c00186

Figure Lengend Snippet: The activation of NK pathway in the EE group. (A–B) KEGG pathway for both up- and downregulated genes. (C) IHC staining was used to detect the expression of CD69. (D) The quantitative analysis of CD69 was performed with ImageJ software. (E–G) q-PCR detected the mRNA expression of NK receptors (H60, RAET, ULBP). n = 5, each group. * p < 0.05, t test. SE: standard environment; EE: environmental enrichment; RAET1: retinoic acid early transcript 1; ULBP1: UL16 binding protein 1.

Article Snippet: The protocol was similar to the IF staining: (1) dewax brain paraffin sections (4 μm) with water; (2) repair antigen and freshen with PBS for 5 min; (3) seal with 5% goat serum for 50 min; (4) add primary antibody CD69 (bs-2499R, Bioss, diluted with 1:200) overnight at 4 °C and wash with PBS for 5 min; (5) add HRP labeled goat antirabbit secondary antibody (GB23303, Servicebio, diluted with 1:200); (6) restain nuclei with DAPI for 10 min; (7) quench tissue autofluorescence; (8) seal sheet.

Techniques: Activation Assay, Immunohistochemistry, Expressing, Software, Binding Assay